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. 2022 Sep 26;50(18):10695–10716. doi: 10.1093/nar/gkac817

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — NOP2/NSUN1 interacts with nuclear and nucleolar snoRNPs. (A) Nuclear and nucleolar extracts from HEK293T cells expressing empty vector (Mock) or FLAG-tagged NOP2/NUSN1 WT were immunoprecipitated (IP) with an anti-FLAG antibody. A fraction of the IP sample was analyzed by western blot. (B) RNA from the remaining IP sample in (A) was extracted and analyzed by RT-qPCR with SNORD3/U3, SNORD118/U8 and U6 specific primers. (C) Nuclear and nucleolar extracts from HCT116 cells were immunoprecipitated with an anti-NOP56 antibody. Associated proteins were analyzed by western blot. Normal rabbit IgG was used as negative immunoprecipitation control. (D, E) HCT116 cells were lysed and immunoprecipitated with anti-RUVBL1, anti-NUFIP1 (D), or anti-La (E) antibodies. Associated proteins were analyzed by western blot. Normal rabbit IgG was used as negative immunoprecipitation control. Asterisk (*) indicates non-specific bands.