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. 2022 Sep 27;50(18):10756–10771. doi: 10.1093/nar/gkac799

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Validation of actionable hit variants. (A) Wild-type (WT) and variants (Var) Kozak sequences of the selected hit genes. (B) Translational enhancement analysed as EGFP/mCherry expression by high content image analysis. The violin plots report the data distribution from n = 3 biological replicates. The dashed line indicates the population median. (C) The histogram represents the mean of the populations analysed by high content image analysis. Data are means ± SD from n = 3 biological replicates. The numbers indicate the percentage of mean increase of the variants over the WT. Statistically significant differences were calculated using the unpaired t-test of each variant versus the corresponding WT.