TABLE 2.
Specificity and species cross-reactivity of the human IFN-γ EIA
| Cytokine source and/or concna | Concn (IU/ml)b |
|---|---|
| Human | |
| rIFN-γ (no treatment) | 174 |
| rIFN-γ (pH 2 treatment [HCl for 1 h]) | 4.7 (97.3) |
| rIFN-γ (heat treatment [56°C for 1 h]) | 0 (100) |
| nIFN-γ (no treatment) | 232 |
| nIFN-γ (pH 2 treatment [HCl for 1 h]) | 3.2 (98.6) |
| nIFN-γ (heat treatment [56°C for 1 h]) | 0 (100) |
| nIL-2 (200 IU/ml) | 0 |
| rIL-4 (5 ng/ml) | 0 |
| rIL-5 (100 ng/ml) | 0 |
| rIL-6 (100 ng/ml) | 0 |
| rIL-10 (100 ng/ml) | 0 |
| rIL-12 (100 ng/ml) | 0 |
| Nonhuman primate IFN-γ | |
| Pan troglodytes (chimpanzee)c | 73.1 |
| Pongo pygmaeus (orangutan)c | 35.9 |
| Hylobates spp. (gibbon)c | 247 |
| Saimiri (squirrel monkey)d | 98 |
| Colobus satanas (guerezas)c | 1.8 |
| Papio sphinx (mandrill)c | 0 |
| Papio hamadryas (sacred baboon)c | 0.1 |
| Macaca fascicularis (cynomolgus macaque)c | 0 |
| Murine rIFN-γ (5,000 IU/ml) | 0 |
| Bovine rIFN-γ (100 ng/ml) | 2 |
a
rIFN-γ and nIFN-γ, recombinant and natural IFN-γ, respectively; rIL and nIL, recombinant and natural interleukin, respectively.
b
Values in parentheses are percent reduction values.
c
PHA-stimulated whole blood.
d
ConA-stimulated PBL.