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. 2022 Sep 26;50(18):10376–10384. doi: 10.1093/nar/gkac790

Figure 6.

Figure 6.

Accessibility of nucleosome protein surfaces indicates two productive RSC-binding orientations. H2B S112C-MB were incubated in the absence (lanes 3–10) or presence of streptavidin (lanes 11–19). Nucleosomes were incubated for 60 min at 30°C with HhaI without RSC (lanes 3 and lane 12, respectively), or without HhaI in the presence of RSC ± ATP (lanes 4 and 5, and lanes 13 and 14, respectively). Additionally, unbound and SA bound nucleosomes were incubated with HhaI, in the presence of RSC and ATP and time points were taken after 0, 10, 20, 40 and 60 min of remodeling at 30°C (lanes 6–10 and lanes 15–19, respectively). Lane 1, free DNA; lanes 2 and 11, unbound and SA bound nucleosomes, respectively. Nuc, 1SA, and 2SA indicate species bound by 0, 1 or 2 streptavidins (see also Supplementary Figure S3).