Skip to main content
. 2022 Sep 26;28(20):4494–4508. doi: 10.1158/1078-0432.CCR-22-0543

Figure 1.

Figure 1. Histopathologic evaluation of the TIME of pretreatment and residual chemotherapy-resistant samples. A, Schematic representing the four-step strategy for evaluation of immune TIME within pre- and post-NAC residual cancers. B, Flow chart of the study population selection. C, Kaplan–Meier curves for PFS in ER+HER2– and TNBC patients with RCB II and III. D, Kaplan–Meier curves for OS in ER+HER2– and TNBC patients with RCB II and III disease. E, Representative H&E images depicting increasing stromal lymphocytic infiltration within residual cancers. F, sTILs levels in pre- (baseline) and posttreatment (RCB II/III) samples within ER+HER2– and TNBC cancers. Statistical significance was calculated using the nonparametric Mann–Whitney U test. The percentage of stromal TILs is the area of stromal tissue (area occupied by mononuclear inflammatory cells over total intratumoral stromal area). G and H, Changes in stromal lymphocytic infiltration in matched pretreatment and residual disease samples. P values from two-tailed paired t tests are shown. I, Kaplan–Meier curves for (i) PFS and (ii) OS in ER+HER2– patients stratified according to sTIL infiltration (cutoff 10%). J, Kaplan–Meier curves for (i) PFS and (ii) OS in TNBC patients stratified according to sTIL infiltration (cutoff 10%).

Histopathologic evaluation of the TIME of pretreatment and residual chemotherapy-resistant samples. A, Schematic representing the four-step strategy for evaluation of immune TIME within pre- and post-NAC residual cancers. B, Flow chart of the study population selection. C, Kaplan–Meier curves for PFS in ER+HER2– and TNBC patients with RCB II and III. D, Kaplan–Meier curves for OS in ER+HER2– and TNBC patients with RCB II and III disease. E, Representative H&E images depicting increasing stromal lymphocytic infiltration within residual cancers. F, sTIL levels in pre- (baseline) and posttreatment (RCB II/III) samples within ER+HER2– and TNBC cancers. Statistical significance was calculated using the nonparametric Mann–Whitney U test. The percentage of stromal TILs is the area of stromal tissue (area occupied by mononuclear inflammatory cells over total intratumoral stromal area). G and H, Changes in stromal lymphocytic infiltration in matched pretreatment and residual disease samples. P values from two-tailed paired t tests are shown. I, Kaplan–Meier curves for (i) PFS and (ii) OS in ER+HER2– patients stratified according to sTIL infiltration (cutoff 10%). J, Kaplan–Meier curves for (i) PFS and (ii) OS in patients with TNBC stratified according to sTIL infiltration (cutoff 10%).