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Chromatographic conditions.
| Sl no. | Retention [min] | Flow [mL/min] | A% | %B | %C |
|---|---|---|---|---|---|
| 1 | 0.000 | 1.000 | 0.00 | 100 | 0.0 |
| 2 | 4.000 | 1.000 | 3.00 | 95.0 | 2.0 |
| 3 | 10.00 | 1.000 | 3.00 | 92.0 | 2.0 |
| 4 | 14.00 | 0.800 | 6.00 | 90.0 | 4.0 |
| 5 | 20.00 | 0.800 | 10.00 | 85.0 | 5.0 |
| 6 | 24.00 | 0.750 | 14.00 | 80.0 | 6.0 |
| 7 | 30.000 | 0.750 | 15.00 | 75.0 | 10.0 |
| 8 | 39.00 | 0.750 | 20.00 | 65.0 | 15.0 |
| 9 | 45.00 | 0.750 | 25.00 | 55.0 | 20.0 |
| 10 | 55.00 | 1.00 | 0.00 | 100 | 0.0 |
Where, A = Acetonitrile, B= Acetic acid solution of pH 3.0, C = Methanol.
