Figure 5.

System immunity activated by PDT‐arousing pyroptosis. A) Schematic illustration of PDT‐arousing pyroptosis by TBD‐3C to a distant orthotopic tumor. C57 mice were subcutaneously and orthotopically inoculated with KPC cells on day 0. PBS or TBD‐3C was then intratumorally injected (subcutaneous) on day 7 and under white light irradiation (40 mW cm⁻², 10 min) 24 h later. B) In vivo bioluminescence imaging of the KPC tumors (both subcutaneous and orthotopic) in control and treated groups. Three representative mice of each group are shown. C) The quantitative bioluminescence signals of tumors in the different groups at different time points. D) The weights of subcutaneous and orthotopic tumors in different groups. Flow cytometry quantification of E) CD8⁺ T cells, F) IFN‐γ ⁺CD8⁺ T cells, G) perforin⁺CD8⁺ T cells, and H) Tregs in the excised tumors collected from KPC orthotopic xenograft model. Statistic results for the proportions of I) M‐MDSC, J) G‐MDSC, K) M1 macrophages, and L) M2 macrophages in the TME of KPC orthotopic xenograft model. Data are presented as the mean ± SD. Statistical analysis was performed by Students’ t‐test (ns, nonsignificant. **p < 0.01 and ***p < 0.001).