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Steps in purification of monoisotypic IgG1 and IgG2 preparations and determination of IgG1 and IgG2 anti-Pnc PS in pneumococcal reference serum 89-SF. Those steps marked with a superscript “a” were followed by determination of IgG1 titer to Tt and IgG2 titer to Pnc PS 6B (Fig. 2) and subsequent pooling of the fractions into subclass preparations.
