TABLE 3.
Effect of excess serum IgG2 anti-Pnc PS on measurement of IgG1 anti-Pnc PS type 6B, 14, 19F, or 23Fa
| IgG2/IgG1 ratio | IgG1 concn (μg/ml) measured by IgG subclass ELISA
|
|||
|---|---|---|---|---|
| 6B | 14 | 19F | 23F | |
| <4b | 3.80 | 10.32 | 6.54 | 5.00 |
| 10 | 3.61 | 10.37 | 6.36 | 4.46 |
| 25 | 3.61 | 10.89 | 6.62 | 4.58 |
| 50 | 4.04 | 11.35 | 7.43 | 4.99 |
| 100 | 4.29 | 12.10 | 5.92 | |
a
Increasing amounts of IgG2-predominating adult sera were added to IgG1-predominating adult sera to produce the IgG2/IgG1 ratios described.
b
No excess serum IgG2 present. Results are the IgG1 anti-Pnc PS concentrations in the IgG1-predominating serum.