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. 2022 Sep 30;12:960858. doi: 10.3389/fonc.2022.960858

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Copyright © 2022 Zhu, Wang, Zhang, Gong, Zhang, Yang, Qu, Wu, Ma, Wang and Qian

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Huaier inhibited pancreatic cancer cell proliferation in vitro and tumour growth in vivo. (A) Bright field images showing the cell morphology of Panc-1 and MiaPaCa-2 cells after treatment with various concentrations of Huaier. (B, C) CCK8 assay showing the viability of Panc-1 and MiaPaCa-2 cells after treatment with various concentrations of Huaier for 24 h, 48 h, 72 h, and 96 h. (D, E) Colony formation assay and its statistical analysis showing the cell proliferation of Panc-1 and MiaPaCa-2 cells after Huaier treatments for 2 weeks. (F, G) IHC staining of Ki67 and its statistical analysis showing the proliferation status of pancreatic cancer tumours in the control and Huaier groups. *P < 0.05, scale bar = 100 μm.