FIGURE 2.

Quantification of microgliosis-, astrocytosis-, cannabinoid type 2 receptor (CB₂R), and Aβ plaque-associated enrichment in 17-month-old arcAβ mice. (A) Representative CB₂R (red) and 6E10 (Aβ, green) staining in half hemisphere of one arcAβ mouse brain. White boxes in panel (A) indicate the regions including cortex (Ctx), cornu ammonus 1 (CA1) region of the hippocampus (Hpc) that zoomed-in in Figure 1. Scale bar = 1 mm. (B) Increased CB₂R (% area) in the Ctx, Hpc, and thalamus (Thal) of arcAβ mice (n = 3) compared to non-transgenic littermates (NTLs, n = 3). (C,D) Increased levels of Iba1 (% area) in the Ctx, Hpc, and Thal and GFAP (% area) in the Ctx and Hpc of arcAβ mice (n = 3) compared to NTL (n = 3). (E,F) Increased CB₂R signal density and mean signal intensity on both GFAP+ astrocytes and Iba1+ microglia of arcAβ mice (n = 3) compared to NTL (n = 3). (G) Increased 6E10 staining of Aβ plaque in the Ctx and Hpc of arcAβ mice (n = 3) compared to NTL (n = 3). (H) CB₂R mean signal intensity on the glia inside plaque is higher than peri-plaque, with low background signal in the parenchymal of arcAβ mice. Data are presented as the mean ± standard deviation.