FIGURE 4.

Comparable regional [¹⁸F]RoSMA-18-d6 and [¹¹C]RSR-056 specific binding in the brains of arcAβ and non-transgenic mice at 6, 17, and 24 months of age. (A) Representative [¹⁸F]RoSMA-18-d6 (0.5 nM) autoradiographic images (total binding) of sagittal brain sections of arcAβ and non-transgenic littermate (NTL) mice at 6 months of age (n = 5, 6), 17 months of age (n = 4, 5), and 24 months of age (n = 5, 5). (C) Representative [¹¹C]RSR-056 (2 nM) autoradiographic images (total binding) of sagittal brain sections of arcAβ and non-transgenic littermate (NTL) mice at 6 months of age (n = 3, 3), 17 months of age (n = 3, 3), and 24 months of age (n = 3, 3). (B,D) Quantification of specific binding of [¹⁸F]RoSMA-18-d6 (0.5 nM), and [¹¹C]RSR-056 (2 nM) in the whole sagittal brain slice in panels (A,C). Two-way ANOVA, arcAβ vs. NTL. (E) Robust correlation between specific binding of [¹⁸F]RoSMA-18-d6 (0.5 nM) and [¹¹C]RSR-056 (2 nM) autoradiograms on arcAβ and NTL mouse brain hemispheres (Spearman rank, r = 0.8042, p = 0.0025). (F) Cnr2 expression in arcAβ and NTL mouse brain hemisphere homogenates at 6 months of age (n = 5, 5), 17 months of age (n = 5, 6), and 24 months of age (n = 5, 5).