Figure 2.

Single cell imaging and analysis of astrocyte volumes 24 h after AIE during withdrawal and 26-days after AIE. (A) Representative images of astrocyte surface rendering from the ACC, mPFC, LO-OFC, and VO-OFC 24 h after the final dose of EtOH. Scale bar, 20 µm. (B) Quantification of astrocyte volumes 24 h after the final dose of EtOH. When comparing astrocyte volume across subregions within the control groups, there was a significant decrease in astrocyte volume in LO-OFC and VO-OFC when compared to ACC and mPFC. There was no significant treatment effect (H₂O vs. EtOH) within subregions. (C) Representative images of astrocyte surface rendering from the ACC, mPFC, LO-OFC, and VO-OFC following a 26-day forced abstinence period. Scale bar 20 µm. (D) Quantification of astrocyte volumes following a 26 day-day forced abstinence period. When comparing control conditions across subregions, there was a significant decrease in astrocyte volume in LO-OFC and VO-OFC when compared to ACC and mPFC. There was no significant treatment effect (H₂O vs. EtOH) within subregions (p > 0.05). Error bars represent minimum and maximum values in each data set. Data presented as box and whisker plots with interquartile range with mean, minimum, and maximum measures indicated along with individual data points. Analysis: two-way ANOVA with Tukey’s post hoc comparison, n = 5/treatment group.