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. Author manuscript; available in PMC: 2023 Nov 1.
Published in final edited form as: Immunology. 2022 Jul 30;167(3):354–367. doi: 10.1111/imm.13541

Figure 5 – Immunophenotype of spleen cells from NZM2410 lupus-prone Cre-CD11c-Floxed-ERα mice.

Figure 5 –

Panel A shows the preliminary gating strategy for isolating immune cells (Time, singlets, debris dump, live/dead staining). Subsequent gating is as follows: pDCs (Lin-, F4/80−, MHCII+, B220+/SiglecH+); cDCs (Lin−, F4/80−, MHCII+, CD11c++, CD11blo); B cells (CD19). All flow experiments utilized FMOs, found in Supplementary Figure 2. Panel B depicts differences in the % live of cDCs and pDCs.