Table 1.

Information for specific loci examined in this study: locus being investigated, size of polymerase chain reaction (PCR) amplicons, primers used in PCR and sequencing methods, and thermal profiles for PCR reactions (all profiles included an initial 2–10 min denaturation cycle). Abbreviations for genes are as follows: small subunit rRNA, 12S; large subunit rRNA, 16S; intron 2 of alcohol dehydrogenase, Adh1-I2; intron 7 of B-fibrinogen, Bfib; cytochrome-c oxidase subunit II, CoII; cytochrome-b, Cytb; exon 3 of engrailed 2, En2; exon 3 of mineralocorticoid receptor, Mlr; and exon 35 and intron 35 of myosin heavy polypeptide 6α, Myh6. References for methods are listed in parentheses. The annealing temperature for the Adh1-I2 reaction was ramped from 53°C to 48°C to −53°C to 73°C at a rate of 0.6°C per second.

Locus	Size	Primers	Thermal profile
			Cycles	Denaturation	Annealing	Extension
12S	531 bp	12Sa (Matocq et al. 2007)	33	94°C, 1 min	62°C, 1 min	72°C, 1 min
		12Sb (Matocq et al. 2007)
16S	571 bp	16Sa (Matocq et al. 2007)	33	94°C, 1 min	45°C, 1 min	72°C, 1 min
		16Sb (Matocq et al. 2007)
Adh1-I2	583 bp	EXONII-F (Amman et al. 2006)	30	95°C, 30 s	53°C to 73°C*	73°C, 4 min
		EXONIII-R (Amman et al. 2006)
Bfib	777 bp	β17-mammL (Matocq et al. 2007)	33	94°C, 1 min	60°C, 1 min	72°C, 1 min
		βfib-mammU (Matocq et al. 2007)
CoII	633 bp	COIIa (Atkins and Honeycutt 1994)	33	94°C, 1 min	51°C, 1 min	72°C, 1 min
		COIIb (Atkins and Honeycutt 1994)
Cytb	1,143 bp	LGL765 (Bickham et al. 1995)	33	94°C, 40 s	51°C, 45 s	73°C, 1 min 20 s
		LGL766 (Bickham et al. 2004)
		700H (Peppers and Bradley 2000)
		400F (Edwards et al. 2001)
En2	146 bp	EN2f (Lyons et al. 1997)	33	94°C, 1 min	57°C, 1 min	72°C, 1 min
		EN2r (Lyons et al. 1997)
Mlr	205 bp	MLRf (Lyons et al. 1997)	33	94°C, 1 min	56°C, 1 min	72°C, 1 min
		MLRr (Lyons et al. 1997)
Myh6	236 bp	MYH2f (Lyons et al. 1997)	33	94°C, 1 min	62°C, 1 min	72°C, 1 min
		MYH2r (Lyons et al. 1997)