Skip to main content
. 2022 Oct 13;17(10):e0275564. doi: 10.1371/journal.pone.0275564

Fig 1. Generation of APRIL-/- and BLyS-/- rodents using CRISPR/Cas9.

Fig 1

(A) BLyS and APRIL genes were deleted for early exons, including the transcription and translation start sites. Shaded in grey are exons that were removed using CRISPR/Cas9 guide sequences. These rats are expected to be genetically null for BLyS and APRIL. (B) Genomic sequencing confirmed the excision sites for both BLyS (Tnfsf13b) and APRIL (Tnfsf13) genes. (C) PCR products from heterozygous BLyS and APRIL knockout rats migrates faster than WT PCR product without deletion. Exon 1 and 2 were deleted from the gene for BLyS leaving a smaller PCR product of the expected size for the deletion. Exon 1 was deleted from APRIL. Genomic DNA was sequenced to confirm the excision sites.