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. 2022 Sep 30;14(19):4794. doi: 10.3390/cancers14194794

Figure 4.

Figure 4

Characterization of ORAI1KO-GCaMP6m-MDA-MB-231 (ORAI1KO) cells. (A) (i) Representative immunoblot showing ORAI1 protein levels in GCaMP6m-MDA-MB-231 (WT) and ORAI1KO-GCaMP6m-MDA-MB-231 (ORAI1KO) cells. (ii) Densitometry comparing the levels of ORAI1 protein in WT and ORAI1KO cells. Statistical analysis was performed using a paired t-test. * p < 0.05 (B) (i) Trace shows Ca2+ influx (CaCl2 addition) following a 690 s pre-incubation with DMSO (unstimulated Ca2+ influx) and CPA (SOCE) in WT and ORAI1KO cells. Bar graphs show the mean ± S.E.M of the ratio of peak 2 (Ca2+ re-addition or SOCE) relative to peak 1 (CPA addition). Statistical analysis was performed using a one-way ANOVA with Bonferroni’s test. *** p < 0.001; ** p < 0.01 (C) Traces show mean [Ca2+]CYT increase as a result of ATP addition in (i) WT and (ii) ORAI1KO cells. Concentration-response curves compare the (iii) peak relative increase in [Ca2+]CYT and (iv) sustained increases in [Ca2+]CYT at 600 s between WT and ORAI1KO cells treated with SC79. Statistical analyses were performed using a two-way ANOVA with a Bonferroni’s test. **** p < 0.0001, ** p < 0.01. Uncropped Western blots in Figure S4.