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SC79-mediated Ca2+ influx is abolished in ORAI1KO-GCaMP6m-MDA-MB-231 cells. Traces show Ca2+ influx as a result of SC79 treatment (1 h) in (i) WT and (ii) ORAI1KO cells. (iii) Concentration-response curve compares the sustained increases in [Ca2+]CYT at 600 s between WT and ORAI1KO cells. Statistical analysis was done using a two-way ANOVA with a Bonferroni’s test. **** p < 0.0001, *** p < 0.001.
