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RT-MCD of di-Fe(III) CjTsdA (black), after anaerobic incubation with 1.5 mM sulfite (red) followed by sufficient ferricyanide to fully reoxidize (blue). The dashed traces are expanded ×10. Samples (see Table S1 for protein concentrations) in anaerobic 50 mM HEPES, 50 mM NaCl, pH 7.
