FIG. 3.

Neutralizing anti-TGF-β monoclonal antibody significantly reverses the inhibitory effect of TGF-β on BCG-induced TNF-α secretion. Cells (10⁶/ml) were incubated with TGF-β in the presence of two different amounts of an anti-TGF-β monoclonal antibody or an isotype-matched control monoclonal antibody (IgG1) for 2 h prior to BCG stimulation for 18 h at 37°C. TNF-α levels were measured by ELISA. The results are the means ± SEMs for seven donors. The values in parentheses indicate percent inhibition by TGF-β with respect to BCG cultures.