Skip to main content
. 2022 Oct 13;13:6036. doi: 10.1038/s41467-022-33780-1

Fig. 1. ScRNAseq of ICC/IDC-enriched and benign-enriched prostate.

Fig. 1

a Schematic of scRNAseq protocol of ICC/IDC-enriched and benign-enriched prostate. b Representative rapid frozen H&E of benign-enriched and ICC/IDC-enriched prostate isolated for scRNAseq at 100x, bar = 200 µm, (n = 7 biologically independent samples). This image and the six additional representative images are in Supplementary Fig. 1. c Patient clinical characteristics. d Representative HMWCK (High Molecular Weight Cytokeratin), TP63, AMACR, AR, ERG, and PTEN expression by IHC on patient FFPE prostate tissue from RP at 100x, bar = 100 µm, (n = 7 biologically independent samples). This image and the six additional representative images are in Supplementary Fig. 2a. e AMACR, AR, ERG, and PTEN expression by IHC per patient. f, g The number (f) and percent (g) of benign-enriched and ICC/IDC-enriched cells per patient. h, i Unsupervised graph-based clustering of all samples visualized by UMAP delineated by cluster (h) and cell-type (i). j Bubble plot of representative cell-type specific markers across all clusters. Source data are provided as a Source Data file.