REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
EGF	Thermo Fisher Scientific	Cat#PHG0311
Horse Serum	Thermo Fisher Scientific	Cat#26050088
Insulin	Sigma-Aldrich	Cat#I9278
Cholera Toxin	Sigma-Aldrich	Cat#C8052
Hydrocortisone	Sigma-Aldrich	Cat#H0888
PBS	Thermo Fisher Scientific	Cat#10010023
Opti-MEM	Thermo Fisher Scientific	Cat#31985070
DMEM/F12	Thermo Fisher Scientific	Cat#11320033
DMEM/F12, no phenol red	Thermo Fisher Scientific	Cat#21041025
Bovine Serum Albumin solution	Sigma	Cat#9048-46-8
FuGENE HD	Promega	Cat#E231A
Puromycin	Thermo Fisher Scientific	Cat#A1113803
Collagen I	Sigma	Cat#9007-34-5
FNC Coating Mix	Athena Enzyme Systems	Cat#0407
Experimental models: Cell lines
HEK 293T	ATCC	CRL-11268
MCF10A	ATCC	CRL-10317
Recombinant DNA
Plasmid: ERKTR-mCherry	Sparta et al. (2015)	N/A
Plasmid: EKAR3-FRET	Sparta et al. (2015)	N/A
Software and algorithms
MetaMorph NX 7.10	Molecular Devices	https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy
ImageJ	ImageJ	https://imagej.nih.gov/ij/
Other
PDMS	Dow Corning	Cat#0008691565
Cover glass	Fisher Scientific	Cat#12-548-BP
Precision glass cutters	Structure Probe Inc.	Cat#07635-AB
3140 Silicone rubber	Dow Corning	Cat#121-353-ML
Biopsy Punch	Integra	Cat#12-460-413
Silver electrode	Advent Research Materials Ltd	Cat#AG549112
True-rms digital multimeter	RadioShack	Cat#2200087