Table 1.
Summary table of the most common single-cell sequencing techniques explored in this section, “Single-cell RNA sequencing”.
Technique | Transcript length | Preamplification | UMI insertion | References |
---|---|---|---|---|
Tang protocol | Full-lenght | PCR | No | Tang et al., 2009 |
STRT-seq | 5' end | PCR | Yes | Islam et al., 2011 |
Smart-seq/ Smart-seq2 | Full-lenght | PCR | No | Ramsköld et al., 2012; Picelli et al., 2013 |
CEL-seq/ CEL-seq2 | 3' end | IVT | Yes | Hashimshony et al., 2012 |
MARS-seq | 3' end | IVT | Yes | Jaitin et al., 2014 |
Drop-seq | 3' end | PCR | Yes | Macosko et al., 2015 |
InDrop | 3' end | PCR | Yes | Klein et al., 2015 |
10x Chromium | 3' end | PCR | Yes | Zheng et al., 2017 |
Smart-seq3 | Full-lenght | PCR | Yes | Hagemann-Jensen et al., 2020 |
FLASH-seq | Full-lenght | RT-PCR | Yes | Hahaut et al., 2022 |
It divides the information according to the steps of this technology: Technique, Reverse transcription, Preamplification, and UMI insertion.PCR (polymerase chain reaction), IVT (in vivo transcription), RT-PCR (reverse transcription-polymerase chain reaction).