Skip to main content
. 2022 Sep 30;13:989464. doi: 10.3389/fmicb.2022.989464

Table 1.

Summary table of the most common single-cell sequencing techniques explored in this section, “Single-cell RNA sequencing”.

Technique Transcript length Preamplification UMI insertion References
Tang protocol Full-lenght PCR No Tang et al., 2009
STRT-seq 5' end PCR Yes Islam et al., 2011
Smart-seq/ Smart-seq2 Full-lenght PCR No Ramsköld et al., 2012; Picelli et al., 2013
CEL-seq/ CEL-seq2 3' end IVT Yes Hashimshony et al., 2012
MARS-seq 3' end IVT Yes Jaitin et al., 2014
Drop-seq 3' end PCR Yes Macosko et al., 2015
InDrop 3' end PCR Yes Klein et al., 2015
10x Chromium 3' end PCR Yes Zheng et al., 2017
Smart-seq3 Full-lenght PCR Yes Hagemann-Jensen et al., 2020
FLASH-seq Full-lenght RT-PCR Yes Hahaut et al., 2022

It divides the information according to the steps of this technology: Technique, Reverse transcription, Preamplification, and UMI insertion.PCR (polymerase chain reaction), IVT (in vivo transcription), RT-PCR (reverse transcription-polymerase chain reaction).