Skip to main content
. 2022 Oct 9;11(19):3164. doi: 10.3390/cells11193164

Figure 2.

Figure 2

Regulation of GR by siRNA and Dex. (A) Silencing of GR by siRNA: Two representative GR Western blots from two patients (P1 and P2, n = 2) show the results of HTPCs transfected with non-targeting (NT) control siRNA and GR siRNA (siGR) at two different time points (48 h and 72 h) each and emphasize the efficiency of the transfection with siGR RNA. (B) Transfection of HTPCs with a GR siRNA abolished the observed effect on IL6 and FKBP5 mRNA. Quantitative PCR data of HTPCs from three different patients (n = 3) transfected with a non-targeting control siRNA (siNT) or GR siRNA (siGR) and treated with Dex (1 µM; siNT + Dex and siGR + Dex) or an equal volume of EtOH (Basal Co and Dex) for 24 h are depicted, ## p ≤ 0.01 vs. Co cells; * p ≤ 0.05, ** p ≤ 0.01 vs. siNT + dex cells. (C) Stimulation of HTPCs with Dex led to the downregulation of GR expression. In the presence of 1 µM Dex for 24 h and 48 h, GR protein in HTPCs from two different donors (P1 and P2, n = 2) was downregulated compared to control (Co), as shown by Western blot. In addition, significantly diminished GR (n = 7; upper left picture) but not AR mRNA (n = 9; lower left picture) was detected after 24 h incubation with 1 µM Dex.