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. 2022 Oct 9;14(19):4950. doi: 10.3390/cancers14194950

Figure 4.

Figure 4

CircKIF4A functions as an miR-1231 sponge in NKTL. (A,B) Levels of the nuclear control (18S), cytoplasmic control (GAPDH), circKIF4A, and KIF4A. (C) MiR-1231 expression in the NKTL cell lines, SNK-1, NK-YS, HANK-1, KHYG-1, NKTL-7, NKTL-16, NKTL-22, and NKTL-29, and in the normal NK cell lines, NK01 and NK02. (D) Predicted miR-1231 binding site in circKIF4A. (E,F) Luciferase assay in HANK-1 and KHYG-1 cells transfected with miR-1231 mimics along with wild type or mutant circKIF4A luciferase reporters. (G) MS2-based RIP assay in cells transfected with MS2bs-circKIF4A, MS2bs-circKIF4A-mut, or control.