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. 2022 Sep 28;14(19):4740. doi: 10.3390/cancers14194740

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Silencing of SNHG1 decreases PPARγ ubiquitination and enhances PPARγ expression via MDM2. (A) The binding of MDM2 to PPARγ detected by IP assay in T24 cells. (B) Western blot analysis of the expression of MDM2 and screening of a shRNA sequence with the highest silencing efficiency. (C) IP assay detecting the change of PPARγ ubiquitination after silencing MDM2. (D) IP assay detecting the change of PPARγ ubiquitination after overexpressing MDM2. (E) MDM2 and PPARγ expression detected by Western blot analysis after overexpressing MDM2 and silencing SNHG1 in T24 cells. * p < 0.05. The cell experiment was repeated three times. Data were presented as mean ± standard deviation and compared by one-way analysis of variance.