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. 2022 Jun 14;8(5):1158–1166. doi: 10.1002/cre2.606

Figure 2.

Figure 2

CGRP is sufficient and necessary for the development of TMJ pathology. Representative histological images of TMJ specimens from young adult wild‐type mice depict (a) normal cytoarchitecture of the condylar articular chondrocytes and joint meniscus harvested from wild‐type mice as detected by Alcian‐Blue/Orange‐G histochemistry (AB/OG). (b) The presence of proteoglycans (purple stain) in the wild‐type mouse TMJ is detected by Safarin‐O histochemistry (SO/FG). (c) Histopathological changes in TMJ articular cartilage (open arrow) and meniscus (closed arrows) resulting from overexpression of the full‐length CGRP following intra‐articular inoculation of FIV(CGRP). (d) The absence of proteoglycans in the TMJ after FIV(CGRP) inoculation (absence of purple staining) compared to control in panel B is noted. (e) Representative histological images of CGRP immunohistochemistry and its cognate receptor in mice with TMJ inflammation. Expression of CGRP in the TMJ meniscus (closed arrows) and (f) CGRP (CLR) receptor expression (open arrows) in articular chondrocytes. (g) Intra‐articular overexpression of the inhibitory peptide CGRP(8‐37) partially alleviated the attendant articular pathology in the TMJ of Col1‐IL1βXAT transgenic mice suffering from TMJ inflammation (Lai et al., 2006) (h). CGRP, calcitonin gene‐related peptide; TMJ, temporomandibular joints.