TABLE 1.
Analysis of serum samplesa
| Sample no. | Reciprocal ELISA titer | Signal ratio | Sample concn (μg/ml)
|
|
|---|---|---|---|---|
| Diluted (dilution) | Original | |||
| 9 | None (negative control serum) | 2.86 | 0 | 0 |
| 7 | <32 | 2.0 | 0 | 0 |
| 8 | <32 | 1.98 | 0 | 0 |
| 3 | 256 | 1.3 | 2.3 (1:4) | 9 |
| 1 | 4,096 | 0.38 | 11.7 (1:4) | 47 |
| 2 | 4,096 | 0.71 | 6.1 (1:9) | 55 |
| 4 | 4,096 | 0.52 | 8.6 (1:4) | 34 |
| 5 | 16,384 | 0.3 | 14.3 (1:29) | 415 |
| 6 | 16,384 | 0.22 | 18.1 (1:19) | 344 |
| 11 | 16,348 | 0.69 | 6.3 (1:29) | 183 |
| 10 | 32,768 | 0.51 | 8.7 (1:29) | 252 |
a
The serum samples prepared at USAMRIID were tested at the Naval Research Laboratory. The samples were tested neat by competitive immunoassay and diluted and retested if necessary to obtain a signal ratio on a responsive portion of the standard curve (Fig. 3). These values are comparable to the ELISA titers determined at USAMRIID.