TABLE 2.
Major applications of CRISPR/Cas technology for DNA and RNA virus resistance in plants
| Virus genome | CRISPR system | Virus family | Virus genus | Virus name | Plant species | References |
|---|---|---|---|---|---|---|
| ssDNA | SpCas9 | Geminiviridae | Mastrevirus | Bean yellow dwarf virus (BeYDV) | Nicotiana benthamiana | Baltes et al. (2015) |
| Curtovirus | Beet severe curly top virus (BSCTV) |
N. benthamiana Arabidopsis thaliana |
Ji et al. (2015); Ali, Abulfaraj, Idris, et al. (2015a) | |||
| Begomovirus |
Tomato yellow leaf curl virus (TYLCV) Cotton leaf curl Kokhran virus (CLCuKoV) Merremia mosaic virus (MeMV) |
N. benthamiana | Ali, Abulfaraj, Idris, et al. (2015a); Ali et al. (2016) | |||
| Begomovirus | Cotton leaf curl Multan virus (CLCuMuV) | N. benthamiana | Yin et al. (2019) | |||
| Mastrevirus | Wheat dwarf virus (WDV) | Hordeum vulgare (barley) | Kis et al. (2019) | |||
| Begomovirus | Tomato yellow leaf curl virus (TYLCV) |
Solanum lycopersicum (tomato) N. benthamiana |
Tashkandi et al. (2018) | |||
| Begomovirus | African cassava mosaic virus (ACMV) | Manihot esculenta (cassava) | Mehta et al. (2019) | |||
| Begomovirus | Chilli leaf curl virus (ChiLCV) | N. benthamiana | Roy et al. (2019) | |||
| dsDNA | Caulimoviridae | Caulimovirus | Cauliflower mosaic virus (CaMV) | A. thaliana | Liu et al. (2018) | |
| Badnavirus | Banana streak virus (BSV) | Musa balbisiana (banana) | Tripathi et al. (2019) | |||
| +ssRNA | Alphaflexiviridae | Potexvirus | Potato virus X (PVX) | S. lycopersicum (tomato) | Wang et al. (2018) | |
| Virgaviridae | Tobamovirus | Tobacco mosaic virus (TMV) | ||||
| Potyviridae | Potyvirus | Soybean mosaic virus (SMV) | Glycine max (soy‐bean) | Zhang et al. (2020) | ||
| FnCas9 | Virgaviridae | Tobamovirus | Tobacco mosaic virus (TMV) |
N. benthamiana A. thaliana |
Zhang, Zheng, et al. (2018b) | |
| Bromoviridae | Cucumovirus | Cucumber mosaic virus (CMV) | ||||
| LshCas13a | Potyviridae | Potyvirus | Turnip mosaic virus (TuMV) |
N. benthamiana A. thaliana |
Aman, Ali, et al. (2018a); Aman, Mahas, et al. (2018b) | |
| dsRNA | LshCas13a | Reoviridae | Fijivirus | Southern rice black‐streaked dwarf virus (SRBSDV) | Oryza sativa (rice) | Zhang, Zhao, et al. (2019b) |
| −ssRNA | LshCas13a | Rhabdoviridae | Cytorhabdovirus | Rice stripe mosaic virus (RSMV) | ||
| +ssRNA | LshCas13a | Virgaviridae | Tobamovirus | Tobacco mosaic virus (TMV) | N. benthamiana | |
| Potyviridae | Potyvirus | Potato virus Y (PVY) | Solanum tuberosum (potato) | Zhan et al. (2019) | ||
|
LshCas13a LwaCas13a BzCas13b PspCas13b CasRx |
Virgaviridae | Tobamovirus | Tobacco mosaic virus (TMV) | N. benthamiana | Mahas et al. (2019) | |
| Potyviridae | Potyvirus | Turnip mosaic virus (TuMV) | ||||
| CasRx | Potyviridae | Potyvirus | Turnip mosaic virus (TuMV) | N. benthamiana | Cao et al. (2021) | |
| Virgaviridae | Tobamovirus | Tobacco mosaic virus (TMV) | ||||
| Bromoviridae | Cucumovirus | Cucumber mosaic virus (CMV) | ||||
|
LshCas13a FnCas9 |
Closteroviridae | Ampelovirus | Grapevine leafroll‐associated virus 3 (GLRaV‐3) | Vitis vinifera (grapevine) | Jiao et al. (2022) |