Figure 2.

AHSA1 promoted the proliferation of HCC both in vitro and in vivo. (A). Transfection efficiency was determined by Western blotting in indicated HCC cell lines. (B,C). EdU detection and corresponding statistical analysis were performed on HCC cells. (D,E). Cell cloning and statistical analysis were performed on HCC cells after knocking down AHSA1. (F,G). Subcutaneous tumor and statistical analysis of tumor volume of HCCLM3-con and HCCLM3-shAHSA1 cells in nude mice. (H). HE and IHC staining of Ki-67 and E-cadherin in subcutaneous tumor tissue. *, p < 0.05; **, p < 0.01; ***, p < 0.001. The uncropped blots are shown in Supplementary Materials.