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. 2022 Oct 13;13:6043. doi: 10.1038/s41467-022-33768-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Experimental design for (b) to (e). Filled circle, control and empty circle, CD4 depleted. b Representative FACS profiles of total TDLN lymphocytes (left) and pre-gated Tcf-1⁺Pmel-1 T cells (right) are shown. c The percentage of Pmel-1 T cells in total TDLN CD8⁺ T cells are shown. d The percentage of Tcf-1⁺ cells in TDLN Pmel-1 T cells are shown. e The percentage of CD69⁺CD103⁺ (left), CD69⁺ (middle), and CD103⁺ (right) in Tcf-1⁺ Pmel-1 T cells are shown. For (c) to (e), n = 5/each. f Experimental design for (g) and h. Black, WT and red, Tgfbr2^−/−. g Representative FACS profiles of donor OT-1 T cells isolated from B16-OVA draining LN (Left) and B16 draining LN (Right) are shown. h The percentage of CD69⁺CD103⁺ cells in stem-like OT-1 T cells (Upper) and the percentage of CD69⁺ cells in stem-like OT-1 T cells (Lower) are shown (n = 5/each). Data are presented as mean ± SEM. Each symbol represents the results from an individual recipient. N.S., not significant (p > 0.05), ****p < 0.0001 and indicated p values are calculated by Student t-test (c–e) and Ordinary one-way ANOVA with multi-comparison posttest (h). Two-sided tests were used. Source data are provided as a Source data file.