Figure 3.

Overexpression of activated SKF restores GluN2B-NMDARs total and phosphorylated levels in PSD sites in HD. The levels of GluN2B (A–D) and P(Tyr1472)GluN2B (E–H) in co-localization with PSD-95, or out of PSD, were evaluated by immunocytochemistry, in proximal and distal neurites, using confocal microscope and Image J software in WT vs. YAC128 striatal neurons. Confocal images were obtained with a 63× objective in confocal microscope Zeiss LSM 710 (scale bar: 10 μm). Data are presented as the mean ± SEM of 4 independent experiments considering 5 to 8 cells per condition per condition. Statistical analysis: ^t p < 0.05, ^tt p < 0.01, and ^tttt p < 0.0001 versus control conditions (nonparametric Mann–Whitney test).