Figure 5.

Overexpression of activated SKF restores CREB-activated levels and reduces caspase-3 levels in HD neurons. The nuclear levels of CREB (A), P(Ser133)CREB (B), and PCREB/CREB (C), as well as the levels of cleaved/active caspase-3 (D) in soma, proximal, (E) and distal (F) neurites were evaluated by immunocytochemistry, using confocal microscope and Image J software in WT vs. YAC128 striatal neurons. Confocal images were obtained with a 63× objective in confocal microscope Zeiss LSM 710 (scale bar: 10 μm). Data are presented as the mean ± SEM of 3 to 4 independent experiments considering ~6 wells/condition. Statistical analysis: * p < 0.05,** p < 0.01, and **** p < 0.0001 versus WT or YAC128 (two-way ANOVA, followed by Sidak’s Multiple Comparison as post-hoc test).