Fig. 3. Overexpression of Coq7 prevents Mn-driven respiratory failure and restores CoQ levels.

a Polarographic quantification of oxygen consumption of WT and ∆pmr1 cells overexpressing Coq7 or harboring the vector control. n = 8. b Survival of WT and ∆pmr1 cells overexpressing Coq7 or harboring the vector control determined via flow cytometric evaluation of propidium iodide staining. Means ± SEM, n = 8. c Metal content of mitochondria isolated from WT and ∆pmr1 cells overexpressing Coq7 or harboring the vector control determined via TXRF. Means ± SEM, n = 3. d Oxygen consumption of WT cells overexpressing Coq7 or harboring the vector control grown in glucose media with or without 5 mM MnCl₂ for 24 h. n = 8. e, f Total cellular CoQ content in lipid extracts of WT and ∆pmr1 cells overexpressing Coq7 or harboring the vector control. Representative chromatograms from 2.5 OD of cells (e) and corresponding quantifications (f) are shown. Means ± SEM, n = 4. g, h Total cellular CoQ content in lipid extracts of WT cells overexpressing Coq7 or harboring the vector control, treated or not with 5 mM MnCl₂ for 24 h. Representative chromatograms from 2.5 OD of cells (g) and corresponding quantifications (h) are shown. Means ± SEM, n = 4. Box plots (a, d) show mean (x), median (line), first/third quartile (lower/upper bound), minimum/maximum within 1.5-fold IQR (lower/upper whisker) and outliers outside 1.5-fold IQR (circle/o). Details for statistical analysis (Supplementary Table 9) and source data are provided.