TABLE 1.
Detection of CEC in blood of patients after FACS or after cytocentrifugation of MNC fractions
| Blood samplea | Amt of blood analyzed (ml)b
|
No. of MNC in MNC fraction/ml of blood (106) | No. of CEC detected
|
No. of CEC/ml of blood
|
No. of CEC/500,000 MNC (Cytospot) | |||
|---|---|---|---|---|---|---|---|---|
| FACS | Cytospot | FACS | Cytospot | FACS | Cytospot | |||
| 1 | 8.0 | 1.9 | 0.43 | 72 | 14 | 9.0 | 7.4 | 17.5 |
| 2 | 4.0 | 0.8 | 1.0 | 43 | 9 | 10.8 | 11.3 | 11 |
| 3 | 5.8 | 0.7 | 0.43 | 13 | 0 | 2.2 | 0 | 0 |
| 4 | 6.0 | 1.0 | 0.1 | 5 | 0 | 0.8 | 0 | 0 |
a
Three blood samples were obtained from a single patient at different time points (samples 1 to 3), and one blood sample was from another patient (sample 4).
b
The amount of blood analyzed refers to the final number of MNC analyzed. MNC were originally obtained from the indicated blood volumes.