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Purified fusion protein Rop2196–561 resolved by SDS-PAGE on 10% acrylamide gel. (A) Coomassie blue staining of the gel. Lanes: M, molecular mass markers; 1, 2 μg of Rop2196–561. (B) Immunoblots of 2 μg of fusion protein Rop2196–561 with an anti-T. gondii-positive human serum (lane 1) and a Toxoplasma-seronegative human serum (lane 2). Molecular masses are given on the left. The sera were used at a dilution of 1:200.
