Skip to main content
. 2022 Sep 28;13:971313. doi: 10.3389/fendo.2022.971313

Figure 2.

Figure 2

Protein overlaps between whole plasma samples and EV-enriched plasma fractions analyzed by LC-MS/MS, and the presence of abundant plasma proteins before and after depletion. (A) Plasma was collected from pre-diabetic female NOD mice. LC-MS/MS analysis was performed on 20 μL of frozen plasma sample or on EVs enriched from 500 μL of frozen plasma by qEV or exoEasy. Venn diagram representing the percentages of shared and unique proteins identified by MS in whole plasma sample (number of proteins = 298), and EV samples enriched by qEV (n = 215) or exoEasy (number of proteins = 356). (B) Plasma was collected from non-diabetic NOD mice, frozen and pooled before analysis. A whole plasma (WP) sample or EV samples enriched using qEV (n = 2, pooled or separate) or exoEasy (n = 2) were either untreated or subjected to removal of three high-abundance/highly-abundant plasma proteins using the Multiple Affinity Removal Spin cartridge mouse-3 column (Agilent Technologies, USA). LC-MS/MS analysis was performed and the percentages of the total protein amount that accounted for albumin, serotransferrin and IgGs in whole plasma (WP), qEV and exoEasy EV samples with (D) and without (ND) depletion is shown (n =1 out of 1-2 for each comparison).