TABLE 1.
Monoclonal antibody combinationsa
| Reagents (FITC/PE) | CD no. (FITC/PE) | Functional identity of relevant subsets |
|---|---|---|
| LeucoGATE | CD45/CD14 | Immunophenotype 3 cell differential (lymphocytes, monocytes, and neutrophils) |
| Isotype control | IgG1/IgG2 | Direct conjugate control for nonspecific staining |
| Leu-4/Leu-3 | CD3/CD4 | CD4+ T cells |
| Leu-4/Leu-2 | CD3/CD8 | CD8+ T cells |
| Leu-4/HLA-DR | CD3/HLA-DR | Activated (MHC class II expression) T-cells |
| Leu-4/Leu-11+ Leu-19 | CD3/CD16 + CD56 | NK cells (CD3− CD16+ CD56+) |
| Leu-4/Leu-12 | CD3/CD19 | T (CD3+ CD19−) and B (CD3− CD19+) cells |
a
FITC, fluorescein isothiocyanate; PE, phycoerythrin; MHC, major histocompatibility complex; IgG1, immunoglobulin G1.