Figure 1.

Combination of antibiotics and doxorubicin reduce tumor growth and lung metastasis formation. (A) Schematic design of murine TNBC model. Image produced by www.biorender.com. (B) Representative images of In Vivo Imaging System (IVIS) of mice after each dose of doxorubicin (Dox) injection over 4-week of treatment. (C) Tumor volume was measured every three days and recorded in mm³. n = 10–15; * p = 0.01, **** p < 0.0001. (D) Tumor weights in grams. n = 9–15; ** p = 0.008. (E) Tumor proliferation marker Ki-67 by IHC immunoreactivity. n = 10–15; * p = 0.01, ** p = 0.001. (F) Apoptosis marker (cleaved caspase 3) in tumors by IHC immunoreactivity. n = 10–11; * p < 0.05, ** p = 0.002, **** p < 0.0001. (G) Representative images of H&E-stained lungs from each treatment group. Lung weights (H), lung lesions (I), and metastatic lesion area (J) measured in the lung. n = 6–15; scale bar = 100 µm; * p = 0.02, ** p = 0.001, *** p = 0.0009, **** p < 0.0001.