Figure 4.

High-fat diet fecal microbiota transplantation (FMT) reduced tumor responsiveness to doxorubicin treatment. (A) Schematic design of murine TNBC model with high-fat diet FMT. Image produced by www.biorender.com. (B) Representative images of In Vivo Imaging System (IVIS) of mice after each dose of doxorubicin injection over 4-week of treatment. (C) Tumor volume was measured every three days and recorded in mm³. n = 6–14; *** p = 0.0001, **** p < 0.0001. (D) Tumor weight in grams. n = 6–14; * p < 0.02, ** p = 0.002. (E) Representative images of H&E-stained lungs from each treatment group. Lung weights (F), lung lesions (G). n = 5–11; * p < 0.05, ** p = 0.005, *** p = 0.0003, **** p < 0.0001. (H) Tumor proliferation marker Ki-67 by IHC immunoreactivity. n = 8–15; ** p = 0.001, *** p = 0.0005, **** p < 0.0001. (I) Apoptosis marker (cleaved caspase 3) in tumors by IHC immunoreactivity. n = 5–15; **** p < 0.0001.