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. 2022 Sep 27;11(19):3021. doi: 10.3390/cells11193021

Figure 1.

Figure 1

C57BL/6 mouse lenses showed progressive increase of ROS accumulation with a significant decline in Bmal1, Nrf2 and its target antioxidant genes expression during aging. (A) Intracellular ROS of lenses of different ages were quantified using H2-DCF-DA dye as described in Materials and Methods and as indicated in figure. Data represents the mean ± S.D. from three independent experiments. Young [6 months (M)] vs. aging (16 M and 22 M) samples. * p < 0.05; ** p < 0.001. (B–L) Total RNA was extracted from lenses of different ages and transcribed cDNA was submitted to real-time PCR analysis with specific primers corresponding molecules as indicated. Data represents the mean ± S.D. from three independent experiments. Young (6 M) vs. aging (16 M and 22 M) samples. * p < 0.05; ** p < 0.001.