Figure 2.

Aging/aged C57BL/6 mouse lenses or hLECs exhibited a significant reduction in phospholipase A2, GSH peroxidase, Catalase and SOD activities. (A–D) Total protein was isolated from different ages of mouse lenses and processed for analyses of the enzymatic activities. Increasing decline of aiPLA₂ (A), GSH peroxidase (B), Catalase (C) and SOD (D) activities were observed in lenses with aging. Data represents the mean ± S.D. from three independent experiments. Younger (6 M) vs. aging (14 M and 22 M) samples. * p < 0.05; ** p < 0.001. (E–H) Primary hLECs isolated from lenses of different ages were divided into three groups: 17–25 y (n = 8); 53–59 y (n = 10) and 65–78 y (n = 10). Total protein was isolated from hLECs of different ages as indicated in figures and was processed for estimating their enzymatic activities. The data represent the mean ± S.D. from three independent experiments. Younger (17–25 y) vs. aging (53–59 y and 65–78 y) samples. * p < 0.05; ** p < 0.001.