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. 2022 Sep 27;11(19):3021. doi: 10.3390/cells11193021

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Metformin provided better cell growth of mouse and human LECs. Cell viability assay showing the concentration-dependent effects of Metformin on growth/health of LECs (A) mLECs line, primary mLECs isolated from C57BL/6 male (B), or female (C), Emb-hLECs (D) and SRA-hLECs (E). Cultured mouse and human LECs were treated with different concentrations of Metformin as shown in figure and were processed for MTS assay to determine nontoxic concentration of Metformin and its effect on cell growth/health. The histograms represent the mean ± S.D. from three independent experiments. Vehicle control vs. Metformin-treated; * p < 0.05; ** p< 0.001.