Table 2.
Diagnostic performance of the quantitative methods included in the study for the detection of animals with focal and diffuse PTB-associated lesions.
| Fecal ddPCR (copies of MAP DNA/μl) | Blood ddPCR (copies of MAP DNA/μl) | ELISA ABCA13 (ng/ml) | ELISA IDEXX (OD) | Fecal qPCR (copies of MAP DNA/gr) | Tissue qPCR (copies of MAP DNA/gr) | |
|---|---|---|---|---|---|---|
| No lesion vs. focal lesion | ||||||
| Cut-off | 2.71 | 23.72 | 3.11 | 3.89 | 84.59 | 2 |
| Sensitivity | 0.48 | 0.71 | 0.53 | 0.68 | 0.15 | 0.46 |
| Specificity | 0.76 | 1 | 0.89 | 0.54 | 1 | 0.8 |
| AUC | 0.59 | 0.78 | 0.63 | 0.54 | 0.57 | 0.61 |
| No lesion vs. diffuse lesion | ||||||
| Cut-off | 19.95 | 3.19 | 2.25 | 19.35 | 7 | 194,809.5 |
| Sensitivity | 0.64 | 0.5 | 0.58 | 0.88 | 0.76 | 0.76 |
| Specificity | 1 | 1 | 0.61 | 0.97 | 1 | 0.8 |
| AUC | 0.8 | 0.62 | 0.49 | 0.95 | 0.88 | 0.78 |
The highest AUC values are presented in bold.