FIG. 5.

Pepscan results obtained with decapeptides of p24 of HIV-1 strain ANT70 with scFv D2 (A) and serum Abs of an HIV-1 patient (B). The numbering on the horizontal axis corresponds with the amino-terminal amino acid of the peptide: the first peptide starts with a Q at −3 relative to the cleavage site of p17 and p24, and the last peptide (no. 240) ends with an A located at −3 relative to the cleavage site of p2 with p7. On the vertical axis, the optical density at 450 nm obtained for each peptide in ELISA has been plotted. The common sequence recognized by the human serum and scFv D2, starting with peptide 76 and ending with peptide 82, is indicated in panel B by solid bars.