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Electrophoretic analysis (10% polyacrylamide gel) and Western blotting of cytoplasmic proteins with MAb RAK-BrI. In a blind experiment, both breast cancers (CA) (3358 and 3218) tested RAK antigen positive and NAT samples tested RAK antigen negative. No RAK antigens were detected in the four breast tissue samples obtained during breast reduction (3600, 3696, 3654, and 3696).
