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Effect of antisense oligonucleotide RAK-I on growth of breast cancer cell line MCF 7. Breast cancer MCF 7 cells were grown for 4 days in the absence (A) or presence (B) of antisense oligonucleotide RAK-I (5′-CCAGACTGTGAGTTGCAACAG-3′), which was added daily to concentrations of 100 μg/ml (day 1) and 50 μg/ml (days 2 and 3). The oligonucleotide (5′-TGTGACATCAGGCTCAAATC-3′) used in control experiments did not affect cell growth (data not shown).
