. 2022 Sep 26;11(19):2996. doi: 10.3390/foods11192996

Table 1.

Primers for PCR detection of LPs biosynthesis genes in B. amyloliquefaciens NCPSJ7.

Gene	Primer	Sequence	Expression Products	Size (bp)	Annealing Tm. (°C)
srfAA	SRFAF	5′-TCGGGACAGGAAGACATCAT	Surfactin	201	58 [11]
srfAA	SRFAR	5′-CCACTCAAACGGATAATCCTGA	Surfactin	201	58 [11]
sfp	SFPF	5′-TATATGGACCGCCCGCTTTCTGC	Sfp ^a	462	60
sfp	SFPR	5′-CCCTTTTCCGGCCTGCTTGATAA	Sfp ^a	462	60
bmyB	BMYBF	5′-GAATCCCGTTGTTCTCCAAA	Bacillomycin	370	55 [11]
bmyB	BMYBR	5′-GCGGGTATTGAATGCTTGTT	Bacillomycin	370	55 [11]
fenD	FENDF	5′-GGCCCGTTCTCTAAATCCAT	Fengycin	269	58 [11]
fenD	FENDR	5′-GTCATGCTGACGAGAGCAAA	Fengycin	269	58 [11]
ituD	ITUDF	5′-TTGAAYGTCAGYGCSCCTTT	Iturin	482	58 [24]
ituD	ITUDR	5′-TGCGMAAATAATGGSGTCGT	Iturin	482	58 [24]
ituC	ITUCF	5′-CCCCCTCGGTCAAGTGAATA	Iturin	594	58 [24]
ituC	ITUCR	5′-TTGGTTAAGCCCTGATGCTC	Iturin	594	58 [24]

^a Sfp, phosphopantheinyl transferase, which is essential for various LPs synthesis [25].