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. 2022 Sep 22;11(19):2960. doi: 10.3390/cells11192960

Figure 1.

Figure 1

Identification of CORK1 through EMS mutagenesis: (A) and (B) Cytoplasmic calcium elevation by 10 µM CT in root (A) and leaf (B) tissue of aequorin wild-type (AeqWT) and two independent, CT non-responsive EMS lines (EMS71) named EMS71-3 and EMS71-11B. Error bars represent SE from at least 10 seedlings. (C) Cytoplasmic calcium elevation by 10 µM CT or 10 µM chitohexaose (chi) in root tissue of AeqWT and EMS71-3. Error bars represent SE from 8 seedlings. (D) Cytoplasmic calcium elevation by 10 µM CT in leaf tissue of EMS71-3 complemented with CORK1 (CORK1-OE) or ARF1 (ARF1-OE). Error bars represent SE from 12 seedlings for AeqWT. Arrows indicate the onset of elicitor application. Statistical significance at the peak value was determined by Tukey’s HSD test with p ≤ 0.05 and is indicated by different lowercase letters. All experiments were repeated at least 3 times with similar results.