Table 2.
Candidate proteins with significant alteration in phosphorylation upon 10 µM CT treatment compared to water control in root tissue of cork1-2 SWT. Numbers between brackets indicate the probability of the modification on the amino acid residue. Calculation for the corrected fold change and the ratio-adjusted p-value is described in the Section 2. SWT: segregated wild-type from the cross to aequorin wild-type. The field ‘Modifications in Proteins’ provides detailed information on the number of amino acid modification of the indicated protein accession number. Phospho: phosphorylation; Acetyl: acetylation; N-term: N-terminus; Met-loss: loss of methionine.
| Comparison | UniProt Accession No. | Annotation | Peptide Sequence | Modifications in Proteins | Corrected Fold Change | Ratio-Adjusted p-Value |
|---|---|---|---|---|---|---|
| 5 min | Q9XIE2 | ABCG36 | RTQSVNDDEEALK | Q9XIE2 1xPhospho [S45(100)] | 10.64 | 1.74 × 10−2 |
| Q9XIE2 | ABCG36 | TQSVNDDEEALK | Q9XIE2 1xPhospho [S45(100)] | 6.97 | 8.76 × 10−3 | |
| Q9XIE2 | ABCG36 | NIEDIFSSGSR | Q9XIE2 1xPhospho [S40(99.4)] | 4.32 | 9.41 × 10−3 | |
| Q9XIE2 | ABCG36 | NIEDIFSSGSRR | Q9XIE2 1xPhospho [S40(99.7)] | 2.50 | 4.26 × 10−4 | |
| Q9FL69 | AGD5 | MESAATPVER | Q9FL69 1xPhospho [T206(100)] | 17.18 | 1.18 × 10−2 | |
| Q9C636 | CC1 | TDSEVTSLAASSPARSPR | Q9C636 2xPhospho [S16(100); S20(100)] | 2.36 | 4.08 × 10−2 | |
| Q9C636 | CC1 | TDSEVTSLAASSPARSPR | Q9C636 1xPhospho [S20(100)] | 2.24 | 2.01 × 10−2 | |
| F4ISU2 | PICC | DIDLSFSSPTKR | F4ISU2 1xPhospho [S1274(99.6)] | 5.71 | 3.55 × 10−3 | |
| F4ISU2 | PICC | SRDIDLSFSSPTK | F4ISU2 1xPhospho [S1274(100)] | 3.28 | 8.22 × 10−3 | |
| F4ISU2 | PICC | DIDLSFSSPTK | F4ISU2 1xPhospho [S1274(99.7)] | 2.80 | 1.89 × 10−2 | |
| Q941L0 | CESA3 | RLPYSSDVNQSPNR | Q941L0 1xPhospho [S176(100)] | 2.80 | 3.76 × 10−3 | |
| Q38868 | CPK9 | AAAAAPGLSPK | Q38868 1xPhospho [S69(100)] | 4.10 | 6.33 × 10−3 | |
| F4IIM1 | CSI1 | MHDSEPPTPHSTTK | F4IIM1 1xPhospho [T37(100)] | 6.28 | 2.56 × 10−2 | |
| Q9FMM3 | EXA1 | VLSSPVVTQASHK | Q9FMM3 1xPhospho [S1553(99.6)] | 4.87 | 3.21 × 10−4 | |
| Q9LUM0 | FAB1B | VAYPVSPALPSK | Q9LUM0 1xPhospho [S1321(100)] | 15.97 | 4.24 × 10−4 | |
| Q9SCZ4 | FER | TGPTLDHTHVSTVVK | Q9SCZ4 1xPhospho [S695(99.2)] | 6.69 | 4.92 × 10−3 | |
| O64851 | IQM4 | FPSPYGPIPSPRPSPR | O64851 2xPhospho [S505(100); S509(100)] | 921.45 | 2.27 × 10−3 | |
| F4JVX1; O64851 | IQM4 | LAYMGIPSPR | F4JVX1 1xPhospho [S520(100)]; O64851 1xPhospho [S525(100)] | 45.79 | 1.31 × 10−5 | |
| Q9FFF6 | JOX2 | SHVESHISPR | Q9FFF6 1xPhospho [S369(100)] | 4.19 | 2.42 × 10−4 | |
| F4HRJ4 | MAPKKK3 | VASTSLPK | F4HRJ4 1xPhospho [T/S] | 2.32 | 1.68 × 10−2 | |
| B3H653 | MPK3 | EATNLIPSPR | B3H653 1xPhospho [S16(100)] | 17.07 | 1.53 × 10−2 | |
| Q39026 | MPK6 | VTSESDFMTEYVVTR | Q39026 1xPhospho [Y223(100)] | 239.27 | 4.67 × 10−3 | |
| Q39026 | MPK6 | VTSESDFMTEYVVTR | Q39026 1xPhospho [T221(100)] | 29.15 | 3.22 × 10−3 | |
| P28187 | RABA5C | QLNSDSYKEELTVNR | P28187 1xPhospho [S186(100)] | 2.21 | 2.47 × 10−2 | |
| Q9FIJ0 | RBOHD | ILSQMLSQK | Q9FIJ0 1xPhospho [S347(100)] | −29.59 | 3.20 × 10−3 | |
| Q94F62; Q94AG2 | SERK1 | DTHVTTAVR | Q94F62 1xPhospho [T450(99.5)]; Q94AG2 1xPhospho [T463(99.5)] | 15.22 | 8.50 × 10−4 | |
| Q39233 | SYP21 | MSFQDLEAGTRSPAPNR | Q39233 1xMet-loss+Acetyl [N-Term]; 1xPhospho [S12(99.2)] | 65.82 | 1.67 × 10−4 | |
| A8MQL1 | TRAF1B | STAVLSSPR | A8MQL1 1xPhospho [S716(100)] | 9.72 | 1.53 × 10−4 | |
| F4KHU8 | UXS3 | QNTTKPPPSPSPLR | F4KHU8 1xPhospho [S31(100)] | 3.13 | 4.96 × 10−3 | |
| Q39160 | XI-1 | AGATGSITTPR | Q39160 1xPhospho [T1195(100)] | 5.97 | 2.51 × 10−2 | |
| 15 min | Q9FL69 | AGD5 | MESAATPVER | Q9FL69 1xPhospho [T206(100)] | 16.41 | 6.12 × 10−5 |
| F4ISU2 | PICC | DIDLSFSSPTKR | F4ISU2 1xPhospho [S1274(99.6)] | 5.71 | 3.55 × 10−3 | |
| F4ISU2 | PICC | DIDLSFSSPTKR | F4ISU2 1xPhospho [S1274(99.6)] | 5.40 | 4.15 × 10−2 | |
| F4ISU2 | PICC | DIDLSFSSPTK | F4ISU2 1xPhospho [S1274(99.7)] | 4.42 | 2.98 × 10−2 | |
| Q38868 | CPK9 | AAAAAPGLSPK | Q38868 1xPhospho [S69(100)] | 5.02 | 1.51 × 10−2 | |
| Q9FHK4 | EDR4 | SLQLEGPGGR | Q9FHK4 1xPhospho [S322(100)] | 5.44 | 7.39 × 10−3 | |
| Q9FMM3 | EXA1 | MTTSSHPPPSPVPTTQK | Q9FMM3 1xPhospho [S1449(100)] | 43.54 | 1.45 × 10−2 | |
| F4JVX1; O64851 | IQM4 | LAYMGIPSPR | F4JVX1 1xPhospho [S520(100)]; O64851 1xPhospho [S525(100)] | 844.25 | 6.30 × 10−5 | |
| F4JVX1; O64851 | IQM4 | LAYMGIPSPR | F4JVX1 1xPhospho [S520(100)]; O64851 1xPhospho [S525(100)] | 99.10 | 1.57 × 10−3 | |
| O64851 | IQM4 | FPSPYGPIPSPRPSPR | O64851 2xPhospho [S505(100); S509(100)] | 355.82 | 3.33 × 10−5 | |
| Q84M93 | MPK17 | LEEHNDDEEEHNSPPHQR | Q84M93 1xPhospho [S397(100)] | 51.33 | 5.67 × 10−3 | |
| B3H653 | MPK3 | EATNLIPSPR | B3H653 1xPhospho [S16(100)] | 196.12 | 3.60 × 10−2 | |
| Q39026 | MPK6 | VTSESDFMTEYVVTR | Q39026 1xPhospho [T221(100)] | 33.63 | 6.00 × 10−4 | |
| Q9LM33 | MPK8 | HHASLPR | Q9LM33 1xPhospho [S495(100)] | −3.12 | 8.53 × 10−3 | |
| Q9LRP1 | NPSN13 | ELKDEEARNSPEVNK | Q9LRP1 1xPhospho [S74(100)] | −184.99 | 1.13 × 10−2 | |
| Q93YU5 | SEC8 | ASQHDINTPR | Q93YU5 1xPhospho [T482(100)] | 88.63 | 1.71 × 10−2 | |
| A8MQL1 | TRAF1B | STAVLSSPR | A8MQL1 1xPhospho [S716(100)] | −3.01 | 2.55 × 10−2 | |
| F4KHU8 | UXS3 | QNTTKPPPSPSPLR | F4KHU8 1xPhospho [S31(100)] | 4.48 | 1.22 × 10−2 | |
| Q9SN95 | UXS5 | QTSPKPPPSPSPLR | Q9SN95 2xPhospho [S15(100); T/S] | 3.13 | 1.18 × 10−2 | |
| P93043 | VPS41 | REDNNRSSFSQR | P93043 1xPhospho [S860(99.7)] | 351.09 | 7.54 × 10−3 | |
| Q96289 | ZAT10 | MALEALTSPR | Q96289 1xMet-loss+Acetyl [N-Term]; 1xPhospho [S8(100)] | 2765.33 | 1.77 × 10−4 |